How is the best way to remove LPS contamination from flagellin extraction?

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State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

Looking for TEM images of osteoclasts under CC BY license: could somebody provide it to me?

I'm looking for TEM images of osteoclasts that I could use in the online Histology Atlas e-Histology (https://e-histologia.unileon.es/Home/index_en.html) under CC BY license or with permission...

27 May 2024 2,735 0 View

Is there a commercially available cell line from human cancer-associated fibroblasts (CAFs) isolated from malignant pleural mesothelioma or lung ca ?

Dear community, I am currently working on a proposal focusing on cancer-associated fibroblasts (CAFs) from malignant pleural mesothelioma (MPM). In my search among commercial cell line providers,...

05 May 2024 5,572 4 View

Does there exist a compact (Hausdorff) topological space X such that if F is a connected closed subset of X, either F=X or F={x} for some x in X?

Clearly X is a connected topological space.

18 March 2024 5,465 10 View

How can the resistance to movement exerted by fine (non-sandy) sediments be calculated?

I am trying to make a simplified model of the movement of meiofaunal animals in marine sediments. Depending on their mode of movement, meiofauna can be classified as either "interstitial" (i.e....

25 February 2024 7,111 1 View

How to Resolve Error in AutoDockTools ?

Hello everyone, I am working with a protein predicted with AlphaFold to which I have added a heme group and optimized using CHARMM-GUI. However, when trying to select it in the AutoDockTools...

17 January 2024 9,426 0 View

How to merge datasets in R by row including only matching records?

Good morning, I have two datasets with the exact same columns. I would like to select rows that have a matching ID between the two datasets (Please, see tables below).I tried to merge datasets...

11 January 2024 1,604 3 View

Can I use glycerol as a preservation medium for plant samples that will be scanned in a CT Scan?

I need to collect plant samples (stems, sores, etc.) to later be scanned in a micro tomograph, and I would like to know if glycerol is a good idea for a preservation medium, or can it generate...

08 January 2024 3,071 1 View

I need to identify this geophilomorph?

The specimen in the photo was captured in Tenerife (Canary Islands). I am sure it is a myriapod of the order Geophilomorpha, but I can't identify the species, could you help me? Thank you very...

19 December 2023 2,329 0 View

How do I map and study changes to river morphology (planforms) through time?

I identified a portion of a small river river (~10km) in my region that went through significant physical changes after a recent forest disturbance, with meanders being cut through and new erosion...

18 December 2023 4,969 9 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

(treating astrocytes with LPS) Has anyone observed the recovery of astrocytes from LPS injury, a few hours after the treatment?

or can you recommend articles showing the differential effect of LPS in a time dependant manner?

28 July 2024 2,547 1 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Are my cells contaminated with mycoplasma?

I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...

11 July 2024 7,786 3 View

Black dots like contamination in my cell cultures. What are they?

There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...

11 July 2024 4,829 0 View

Does the soil contamination correction factor for decomposing leaf biomass vary according to space and season?

The question refer to leafy biomass decomposition using litterbag techniques.

11 July 2024 2,078 0 View

When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?

07 July 2024 5,480 2 View