How is the best way to lable TFH cells for flow cytometry?

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How do I determine the mg of ascorbic acid equivalent to 1.0 mL of standard indophenol solution?

Hello everyone. Could you help me with the following problem. I am going to determine ascorbic acid in capsules using an AOAC method. The method gives the following equation: mg ascorbic...

24 June 2024 789 1 View

Metodology for dashboard, please?

Please scientific articles on methodologies to create dashboards in R shiny and PowerBI

27 May 2024 387 1 View

What are the UV ranges and temperatures mostly effect on microplastic degradation?

For a study about the effect of UV and temperature on plastic degradation and to compare the changes between different UV and temperature levels by using fresh plastic pellets. What would be the...

22 March 2024 4,067 1 View

Do you have seed trait data for tropical species?

We would like to invite you to build an inclusive, open-access database on seed traits for tropical species. If you have morphological, biophysical, or germination data for tropical species,...

07 March 2024 2,897 4 View

Você trabalha com traços funcionais de plantas de Cerrado?

Nós gostaríamos de te convidar para participar e contribuir com com o Cerrado Plant Traits: uma rede para a construção de um banco de dados de traços funcionais de plantas. Se você tem dados...

27 February 2024 2,142 2 View

DDS and Pharmaceutical industry. Conferences opinions?

Hello everyone! I am considering the possibility of assisting Congress or Conferences on Drug Delivery Systems or Pharmaceutical Formulation. However, I do not have much experience in this...

30 January 2024 7,440 0 View

A good anti-GR antibody for western blot in plants?

Hello, I would like to perform a western blot to detect a protein fused to GR (Glucocorticoid Receptor) and expressed from a 35S promoter in transgenic apple plants. I have never used anti-GR...

17 January 2024 7,806 0 View

Independant T-test Vs Paired- T Test results?

What if the Paired T-Test results are significant, but Independent T Test results are not significant? (Done in the same sample) Ex: Paired T-Tests done in the Experimental groups are...

12 January 2024 3,288 4 View

¿Te interesaría hacer divulgación en YouTube? Interested in doing outreach on YouTube?

Estimados Colegas Disculpen las molestias. Estoy intentando hacer divulgación científica por medio de un canal de YouTube –se llama Datos y Ciencias Sociales, el link es...

20 December 2023 3,340 1 View

Abaqus error rfm_ArrayRepr::rfm_ArrayRepr when reading .ODB file?

Dear colleagues, I have a python script to read results from ODBs in Abaqus. More specifically, I am extracting the equivalent plastic strain PEEQ from different regions (sets previously...

28 November 2023 3,311 4 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

How to find projects for a team of professional developers in AI?

We are looking for projects for a team of professional developers About us We are a team of experienced developers with over 10 years of experience in various programming fields. Our mission is to...

30 July 2024 8,377 0 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Technology developments may it for whatever reasons developed eventually its benefits are multi-Disciplinatary?

Your comments

28 July 2024 2,822 2 View