I tried to expand the ecoli-core model attached below.I wanted to change it in a way that the organism consumes Co2 as carbon source and sulfate as energy source.To achieve this goal I removed the ATP producing reactions from the model ( there were 2 reactions) and replace a reaction which produces ATP from sulfate and and Calvin cycle to produce glucose from CO2.When I performed FBA By CobraToolbox,Calvin cycle's reactions worked properly but the flux of ATP production from Sulfate was zero.To solve this I make the reactions which could reversibly produce ATP irreversible so that they can not produce ATP anymore.But even in this case it shows growth,Is it possible to have growth without ATP?I wanted to relate Sulfur metabolism to central meabolism by the help of ATP producing reaction,But If this models continues to grow without ATP there is no way to attach these two parts of metabolism.
Thanks for your answer.As you said I use the E.coli core from gcrg.ucsd.edu/Downloads/EcoliCore,I actually make these for reactions irreversible and replace my favorable reaction which produce ATP from a sulfur compound in this case the added reactions have non zero flux but growth yield is zero I think making those 4 reactions irreversible prevent the network from producing needed precursors and that is why there is no growth.But I don't know how to solve it