(The sample that has been diagnosed mutant; definitely its product has mutant components, because the primer is a component of DNA!)
If you are saying that the dna was amplified with 2 primers one of which has a nucleotide change in its sequence then all amplimers will end up with the change incorporated into the molecule. It can then be sequenced using the pcr primers bearing in mind that you will get no useful sequence under each primer or for the next 30 bases so to get the whole sequence you will need to sequence from both ends ( using both primers separately in 2 sequencing reactions
Agree with @ Paul Rutland
But I think the question needs to be clarified more
However, if a piece of DNA is amplified, the volume information and using the parameters can be first defined by the blast and then the mutations are determined when compared
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