How inflamme dental pulp stem cells with LPS?

More Sepide Sarfi's questions See All

Child process exited abnormally in fsl?

hello. I am using fsl (fslvm7) on vmware workstation on win10 for preprocessing resting state fmri. the bet file is obtained correctly. but in feat , I face the following problem: child process...

11 July 2021 1,446 0 View

Could you help me to find a new factor in transport mode choice in which I can evaluate that factor's influence on mode choices in my model?

I am developing an agent based model about citizen's decision making of daily mode choices which I have already 2 variables that affect the choice of people and I am looking for a new factor to...

14 April 2019 4,561 3 View

Can i use MCF-7 cells and transfect them with miLet 7b to check for regulation of KRAS gene?

I wanted to do experiments on miRNA and its regulatory effects on target oncogene. miRNA Let7 family (7b in particular) has a negative regulatory effect on the KRAS gene. I want to prove this...

05 April 2016 389 3 View

Can I use MODIS data to predict crop harvest date with good precision?

I would like to monitor and estimate harvest date of crop with MODIS. Can I do this work? How is the precize of this work? How many hectares I have to choose for use of MODIS?

22 February 2015 5,033 4 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

How does social media influence people's dental habits and interest in aesthetic dental treatments?

Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...

01 August 2024 9,349 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View