After ethical slaughter a 10-20 gram piece of long bone can be collected from the broiler bird. After initial heating it in a 25 or 50 ml silica crucible to remove moisture, it can be ashed in a muffle furnace and cooled in dessicator. Of course you have take initial and final weights. Calcium can be estimated by Talapatra (1940) method and Phosphorus by spectrophotometric method