Rotavirus is a nonenveloped, segmented RNA virus. I'm working on detecting Rotavirus through rtPCR from stool samples collected from children having diarrhoea. I expect suggestions from experts, on any method of RNA extraction in the absence of commercial extraction kit.
Junjie Shao
do you have trizol?
if you have, you also need order some carrier RNA, to get the viral RNA.
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Md. Sahidur Rahman
Thank you for your information. Would you please give me any link to the full procedure of RNA extraction using trizole and carrier RNA? Junjie Shao
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Junjie Shao
no protocol, but works,
just according to the protocol of trizol and adding carrier RNA before precipitation.
the concentration of Carrier RNA you could check commercial kit.
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