I usually use the H2DCF-DA assay but I would like to try another method because I have strange results and I would like to confirm with another test.
Hello Elisabet,
As an alternative you might use the mitoSOX staining. You can analyse your cells after staining by flow cytometry.
Best,
Hamadi
Select your probe depending of your aim.
https://www.thermofisher.com/by/en/home/references/molecular-probes-the-handbook/probes-for-reactive-oxygen-species-including-nitric-oxide/generating-and-detecting-reactive-oxygen-species.html
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