I need to transduce HL-1 cells and keep them for 5-7 days for the transgene to be expressed but they proliferate very fast, get over-confluent and die around day 3. Etoposide was very toxic for this cell line and decreasing serum concentration did not help. Any idea how I can stop/slow down the cell proliferation?
Hi Faranak,
May I ask what transduction system you are using, lentiviral vector?
It's not so good an idea to introduce extra intervention during experiment, especially interfering with the basic life cycles, such as cell cycle.
Therefore, I would recommend that you may just adopt better transduction methods to improve the transduction efficiency and then sorting the postive out.
You can seed HL-1 cells with medium serum-free. But I advice you to assess if this protocol change will influence your experiment. In all case, you shloud consider the option given by Dr. Zhou
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