10 October 2017 0 3K Report

I'm purifying the protein.

In many cases, targets have basic pI such as pH 8 or 9, but in my case, my target has pH 5 as the pI value.

So, most of strategy didn't work to remove HCP.

Also, I tired IEX or HIC resin, but it also didn't work.

Is there any solution for removal of HCP when target protein and HCP share their pI value?

Thanks.

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