I'm purifying the protein.
In many cases, targets have basic pI such as pH 8 or 9, but in my case, my target has pH 5 as the pI value.
So, most of strategy didn't work to remove HCP.
Also, I tired IEX or HIC resin, but it also didn't work.
Is there any solution for removal of HCP when target protein and HCP share their pI value?
Thanks.