Palan Lee

3 Questions 3 Answers 0 Followers

Questions related from Palan Lee

How I can remove HCP?

I'm purifying the protein. In many cases, targets have basic pI such as pH 8 or 9, but in my case, my target has pH 5 as the pI value. So, most of strategy didn't work to remove HCP. Also, I tired...

10 October 2017 2,856 0 View

The reason of the low peak and low recovery in Sepharose Q FF

Hi, I'm in bioindustry company. We use Sepharose Q FF for purification of our target protein. But recently, the peak was lower than before and the recovery also decreased. I think all of the...

06 June 2017 5,938 6 View

Is there any method cutting leucine binding?

Hello. I have a problem with a protein GLP-2. When I run SDS-PAGE, there are always dimer form of GLP-2. I read the paper about GLP-2 dimerization and it show the reason is 17 leucine. When they...

04 April 2017 5,150 2 View