XRF analysis of 45s5 analysis?

I've sent my synthesized 45s5 powder and a commercial 45s5 powder to XRF analysis but in both of them the amount of Na2O is about 15 percent and LOI is around 40 percent and it makes the aount of...

29 January 2021 239 4 View

No title

I want to simulate a rough surface in Abaqus CAE by transferring nodes in the input file but I have problem in large number of nodes transferring. so I have following steps to pre-process: first...

13 January 2021 8,417 2 View

Sol-gel synthesis of 45S5?

I've synthesized 45S5 bioglass by sol-gel method and my XRD and FTIR results are similar to 45S5 bioglass but my EDX result has differences and instead of Si and Na the amount of Ca is higher....

29 December 2020 4,847 2 View

Sol-gel Synthesis of hydroxyapatite?

I use Ca(NO3)2.4H2O and P2O5 as precursors and Sol-gel method to synthesis my powder but the CA/P ratio is more than stochiometric (167) and my powder is dark. Does anyone know why this happens?

27 December 2020 1,574 5 View

Advance Manufacturing Technologies?

I am looking some research papers about Advance Manufacturing Technologies.

07 December 2020 2,152 5 View

Could anyone help me with this error Exception has occurred: ValueError?

I have downloaded monthly mean wind speed of terraclimate data for 20 years (2000-2019) which have NetCDF format, but unfortunately, each year has a single NetCDF file, and I want to merge them...

25 November 2020 8,477 2 View

Has anyone used Breaks For Additive Season and Trend for decomposing the time series into trend,and season in R package?

12 September 2020 1,513 3 View

How show NDVI MODIS index (MOD13A1) in Arc GIS?This collection of MODIS has calculated NDVI index and i want to show this index in my study area?

This collection of MODIS has 2 options for calculating NDVI index. First, we can calculate it from NDVI formula or we can use NDVI index that MODIS has calculated. I use both of them but they are...

25 August 2020 2,419 3 View

Has anyone used Maximum likelihood or Support vector machine (SVM) for image classification?

Hello I'm working on a project that I want to detect forest and deforestation areas. I need some information about Maximum-likelihood or Support vector machine (SVM) for image classification.

22 August 2020 1,896 3 View

How we can use the Transmisivity of light colors (rtn, gtn, and btn) in simulating a coated glass with red dyes in Radiance?

We want to simulate a red coated glass in Radiance. The UV-VIS spectroscopy analysis was conducted and optical characteristics of the red glass including transmittance and absorbance were...

16 March 2020 3,492 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View