I need a viral lysate. There are some substance to it? Something that disrupts the lipid envelope.
Guanidine Thiocyanate. Have a look at Qiagen's QIAamp mini viral RNA kit, the AVL buffer contains GT which is chaotropic and denatures cellular and virus membrane / envelope proteins.
We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....
17 June 2024 1,649 0 View
In my new research, about TiAlN thin films, i constantly use the word "shift". I would like to know if i can write "displacement" as a synonym, with the intention to not repeat the words.
02 June 2024 9,694 4 View
Good morning, I am running some Steered molecular dynamics in Amber 22 and want to set some restraints in the z-axis so as to imply certain distances from my ligand to its substrate. My goal is...
27 May 2024 7,082 1 View
Good afternoon, We have recently started running ORCA calculation in a new machine, however we are consistently getting memory errors in analytical frequency calculations. Our jobs com-prise a...
27 March 2024 8,609 2 View
We addressed such a question at Exploring The Missing Link Between Trust in Business Negotia...
14 March 2024 2,875 1 View
Hi, I've been etching Ti3AlC2 MAX phase in the lab for a year, having made several batches. In the last 3 I've been observing a peak at around 44°. This peak is observed for multilayer and...
11 March 2024 5,920 0 View
I'm doing a protocol in which I plate my cells in a 12-well plate with complete medium (i.e. DMEM/F-12 WITH 5% FBS), let them reach ~80% confluency and then I change the complete medium for a...
10 January 2024 8,232 5 View
For calculating summary effect size in meta analysis do i need to group correlation/beta coefficient between 2 variables which are significant or even non significant ones. Eg I have 20 studies...
11 December 2023 1,532 2 View
Zoological Record today belongs to Clarivate and at their website, when I click at "Go to product" I am directed to Web of Science basic search. I did not find any filter for Zoological Record...
03 November 2023 8,752 1 View
I've been trying the method described in : Reliable Exfoliation of Large-Area High-Quality Flakes of Gr... The process I'm doing now is: Clean the SiO2/Si substrates with acetone, IPA and DI...
01 November 2023 9,236 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View