in this work i use the standard protocol in extraction& good sampling and use chloroform and twice wash with ethanol 75% but i have phenol contamination (low 260/230 ratio)
for example: OD: 765.4
260/230 : 0.6
260/280 : 1.8
Omar Mohammed Esmaeel
You can try converting the phenolic contamination into counter salt using sodium hydroxide. Otherwise, try to add a reagent that reacts with the phenol but not with the other components.
0 votes
0 thanks
Mateo Glumac
The easiest solution would be to do more chloroform washes. Chloroform dilutes phenol and 2 washes after precipitation with ethanol should be enough. You should precipitate with ethanol after each wash so you do not lose your sample.
0 votes
0 thanks
Chinaza Godswill Awuchi
Kindly check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406948/
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Extraction
More Kamran Javidi's questions See All
Similar questions and discussions