Saeid Mohammadparast

4 Questions 9 Answers 0 Followers

Questions related from Saeid Mohammadparast

How I can do RNA isolation from a single embryo of Zebrafish at day 1 and 2 post fertilization?

Usually we do it with 6 embryos. Now for a lot of reasons we need to do RNA isolation and even Western blot with only a single embryo. Please kindly guide me with your notes. Thanks

16 November 2016 797 4 View

I know the basics of four common Protien-protein interaction methods listed below. Which one is easy to use and provides more reliable results?

I know these methods, but if you know something else and it could be useful, please leave me your pithy notes. regarding this fact that I don't know potential prey proteins, advise me which one I...

20 November 2015 9,744 11 View

Assuming to investigate the role of a gene in mouse model, we need to knock it out spaciotemporally. What is your suggestion of a suitable method?

Consider, that the ablation of this particular gene is lethal for mouse embryonic development. There is no way to use CRISPR/Cas9? I was thinking about Cre-LoxP! Please kindly grant me your...

05 November 2015 4,132 4 View

Could anyone introduce me to a good software for haplotype analysis?

I couldn't use Haploview.

15 May 2013 5,217 1 View