Hadi Bayat

3 Questions 34 Answers 0 Followers

Questions related from Hadi Bayat

What is the best consideration for sgRNAs offset in knocking-out approach by Cas9n?

Dear all, I want to disrupt gene expression by applying Double Nicking by RNA-GuidedCRISPR Cas9. What should be considered during designing guide RNAs, especially sgRNA offset? Thank you in...

17 November 2014 4,191 5 View

Does anyone have experience with forcing modification at DNA binding site of protein?

Dear all, I want to know, Is there any bioinformatical analysis for considering protein 3rd structure by forcing desired modification at DNA binding site? In fact, I am going to make some...

31 July 2014 2,402 5 View

Do you have a protocol for real-time PCR application?

I want to apply a real-time PCR for finding unknown mutation. I was wondering if anyone has a suitable protocol. Thanks in advance.

28 January 2014 7,296 7 View