Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Can I make mixture of cinnamic acid with morin flavanoid ?

I am working on a project for assessing the Antibacterial activity of various chemicals, I was wondering Can i make solution of those components using water as a solvent, depending on the fact...

19 November 2020 7,857 4 View

Did anyone used microflow imaging to measure the size distribution of Multivesicular liposome which are charcterized with size (>3um)??

I have prepared Multivesicular liposomes using the following procedures: Multivesicular liposome formulations for the sustained deliv... Multivesicular liposome (MVL) sustained delivery of a...

18 November 2020 3,189 2 View

How antibodies differentiate according to match every epitope?

How antibodies differentiate according to match every epitope , ie: how takes its shape as a monoclonal

10 October 2020 9,573 2 View

I have 4 latent variables and each of which has 7 observed variables in SEM, how this affects the parsimony of the model?

I am conducting an investigation using SEM (Structural Equation Modelling) and I have 4 latent variables and each of which has 7 observed variables. How this affects the parsimony of the model?...

07 September 2020 718 3 View

How to express Nano-fluid in conservation equation of Mass, Momentum and Energy?

How to express Nano-fluid in conservation equation of Mass, Momentum and Energy?

07 September 2020 5,903 1 View

How to examine hypothesis in SmartPLS?

Should I report the Path coefficient or Latent variable correlations after bootstrapping as the P value and T-Statistics in the latent variable correlations of two hypotheses are met while in path...

07 September 2020 6,994 2 View

I followed a common procedure to synthesize Fe3O4 nanoparticles. The problem is that XRD pattern does not show any crystal structure. Could you help?

Besides, I have performed different characterization tecniques to my Fe3O4 nanoparticles and all are similar to what is reported in literature. Those characterization techniques include FTIR, SAED...

22 July 2020 8,549 12 View

How much water does each desalination plant type produce and cost?

There are many types of water desalination plants; Reverse Osmosis, MSF, Solar desalination units, ........... etc. How much water does each desalination plant type produce and what is the...

30 May 2020 7,719 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View