I have estimated the amount of ammonia liberated using the Nessler method, but now how to calculate L-GLutaminase enzyme activity?
Is there any formula?
What is the formula (equation) to calculate L-glutaminase activity (U/ml) using Nesslerization?
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363723/,u can check this link.
Dear Fenil Parmar . Specific enzyme activity (usually stated simply as 'specific activity') is the number of enzyme units per ml divided by the concentration of protein in mg/ml. See the link: https://www.biomol.com/resources/biomol-blog/guide-to-enzyme-unit-definitions-and-assay-design
Also, specific activity = enzyme units / (vol. in µl x (protein conc. in mg per ml / 1000)). See the link: https://www.mvls.gla.ac.uk/Teaching/SLS-GMC/enz2.html
Total enzyme activity is obtained by calculating the dilution ratio of your assay. The specific activity is the ratio of the enzyme activity divided by the protein concentration fo your enzymatic assay. It si typically expressed as mol of dioxygen consummed per sec per mg of protein. See the useful link: https://www.researchgate.net/post/How-can-I-calculate-Enzyme-activity-Specific-activity-and-Relative-activity-of-an-Enzyme-from-OD
https://www.researchgate.net/figure/The-effect-of-L-glutamine-concentration-on-L-glutaminase-production-from-Hypocrea_fig1_235879078
Thank you for all the valuable inputs, it is really helpful for me...
Prem Baboo, Jaydip Datta, Aref Wazwaz Faraed Salman
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Enzymology
- Enzymes