How fresh the sample for Nanopore should I use?

More Vinna W Putri's questions See All

Good day!! All, can anyone recommend the best battery related journals to download research articles please?

I am an Mphil research student and looking for high impact factor battery related journals to download and read research articles to improve my knowledge and get new ideas in the field of battery...

01 August 2024 6,516 2 View

Why is there overgrowth on LB media after transformation?

I've been doing transformation several times with DH5. I got overgrowth/bacterial lawn growing on my plates twice, and when I transform for the 3rd time, there's no colonies formed. I'm using heat...

22 June 2024 4,843 1 View

Analysis method of ammonium bisulfite (as active substance of oxygen scavenger) ?

Good Day! Thank you for the opportunity, I would like to ask you. Is there any standard method (ASTM or API) for analysis of ammonium bisulfite (as active substance of oxygen scavenger) ? Thank...

29 January 2024 1,926 0 View

Hello, i am currently synthesizing molecularly imprinted polymer with curcumin as a template, which extraction methods can i use to remove a template?

Actually, i have already tried several extractions methods using 10% acetic acid slution in methanol, such as maceration, refluks, and sonication, but the template does not extract, so i hope...

17 January 2024 5,220 2 View

Why do my DH5a ecoli colony looking weird?

Usually, my ecoli colony has a distinct shape and doesn't form a hairy look like this. I use LBA with AMP 100 µL/mL. The plasmid inside my ecoli has the AmpR region so they should survive under...

10 January 2024 9,531 2 View

How to remove an ice bridge in a superconducting magnet?

Our Thermo LTQ FT magnet has an ice bridge between the Helium and Nitrogen sections which causes it to need more and more Helium. Is there a way to remove this ice bridge without shutting the...

09 January 2024 9,319 2 View

What is the theoretical reason behind the exact number of research participants for interview in qualitative descriptive?

i am currently doing a thesis of qualitative descriptive research about students computational thinking ability. based on other research that i read, they categorized the student's ability into 3...

04 December 2023 5,140 2 View

5hmC Enrichment Kit/Protocol?

Hey, I'm looking to find an enrichment kit for 5hmC. I've done a bunch of googling and there are plenty of quantification or detection kits (for example Thermo's EpiJET 5-hmC) but I'm...

30 October 2023 6,691 0 View

How can i correct journal article information?

change No. of paper, etc

10 October 2023 5,172 1 View

When doing DNA extraction, what are the advantages of using Proteinase K over guanidine hydrochloride?

For many years I've been using a simple solution of guanidine hydrochloride and water as my lysis buffer when doing DNA extraction (both for arthropods and fungi). It's always worked fine for me...

01 October 2023 7,329 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Sabine Strehl

This depends on the quality and storage conditions of the samples. It’s crucial to perform a quality check before sequencing to assess the integrity of the DNA.

Damilola Odumade

It depends on the buffer that was used in storing the DNA as well as temperature. DNA samples that have undergone repeated thawing and freezing may not be ideal.