31 October 2023 0 7K Report

Hey,

I'm looking to find an enrichment kit for 5hmC. I've done a bunch of googling and there are plenty of quantification or detection kits (for example Thermo's EpiJET 5-hmC) but I'm specifically looking for a kit (or a protocol) that does enrichment of 5hmC.

Active Motif has a hMeDIP kit but that requires an overnight step and uses Phenol/Chloroform for the purification step, overall I don't find that kit applicable to my needs.

Zymo used to have a kit that would be perfect, the Quest 5hmC Detection Kit. Unfortunately they no longer make this and only have an enrichment kit for 5mC. I've contacted Zymo and they don't have any info on how I could enrich 5hmC.

If anyone has a kit or a specific protocol please send it my way.

Thanks

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