Promoter:GFP (or GUS) lines are used to reveal the expression pattern of genes. But can they also be used in short term treatments? As they are fused to the promoter only, the GFP is not susceptible to regulatory breakdown mechanisms, hence fi in the root of Arabidopsis, a GFP coupled to a root specific promoter will constantly be transcribed and translated for days. If you then (after several days) apply a short treatment, you will see merely the accumulated GFP of the past days, instead of the effect of the treatment (which is too short in comparison with several days to add reasonable amount of GFP proteins)

Hence, my question is: how fast are GFP and GUS broken down in the root of Arabidopsis?

Thanks