Jolien De Lepeleire

5 Questions 3 Answers 0 Followers

Questions related from Jolien De Lepeleire

How fast is GFP (and GUS) degraded in a promoter:GFP (GUS) line?

Promoter:GFP (or GUS) lines are used to reveal the expression pattern of genes. But can they also be used in short term treatments? As they are fused to the promoter only, the GFP is not...

21 November 2018 3,416 1 View

Is it possible to lose genes from constructs during cloning using restriction enzymes?

Hi, Im making a triple gene construct. In the final cloning step, inserting the third gene in the double gene construct, I seemed to have lost the second gene.. Ofcourse, those enzymes to insert...

09 December 2016 6,874 2 View

During RNA extraction (using RNeasy kit), is an on-column Dnase treatment useful when you also performe DNAse treatment (Invitrogen) afterwards?

To be sure to get rid of all DNA I usually do both, but some say that it is useless, because one of the treatments should already remove everything. Still I think that to be sure its better to...

10 April 2015 1,361 4 View

Are there any tips for obtaining clear images of cell files in each zone of Arabidopsis roots?

Are there any tips for obtaining clear images (20x) of cell files in each zone of Arabidopsis roots? I normally fix them first in 96% ethanol and mount them in chloral hydrate. However, due to the...

13 October 2014 3,585 7 View

Can anyone suggest a tool which allows the follow-up of the early development of Arabidopsis roots whilst allowing short term treatments?

Does anyone have an idea of a kind of tool which allows the follow-up of the early development of Arabidopsis roots whilst being able to submit short term treatments? For example by changing the...

02 October 2014 9,519 8 View