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Hi, Im making a triple gene construct. In the final cloning step, inserting the third gene in the double gene construct, I seemed to have lost the second gene.. Ofcourse, those enzymes to insert...
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To be sure to get rid of all DNA I usually do both, but some say that it is useless, because one of the treatments should already remove everything. Still I think that to be sure its better to...
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Are there any tips for obtaining clear images (20x) of cell files in each zone of Arabidopsis roots? I normally fix them first in 96% ethanol and mount them in chloral hydrate. However, due to the...
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