Roy Ehling

3 Questions 2 Answers 0 Followers

Questions related from Roy Ehling

How efficient in Knock-in genome editing in murine HSPCs?

Based a few hours search, there is to date no established protocol for transfecting murine HSPCs for generating knock-ins of several kb size with reported efficiencies. All I can find is...

06 June 2019 3,452 1 View

What exactly happens to the unused J (and V, D) segments of B cells after successful recombination? Are they still present and silenced or removed?

s. above

09 September 2015 9,436 0 View

What do you use to determine the species of your bacterial contamination?

Also, is this cell debris or bacterial contamination?Hybridoma cells with oddly shaped rods in between. They do seem to increase in number, but medium hasn't turned yellow yet and it has been a...

06 June 2015 9,219 6 View