Which goat breed is best adapted in the pacific island countries against climate change?

Like Marwari goats are well adapted against climate changes in India. I would like to know about PIC's?

16 April 2019 9,063 1 View

How we can able to manage the role of middleman in buying and selling of Agricultural Commodities?

How we can able to manage the role of middleman in buying and selling of Agricultural Commodities?

20 February 2019 3,025 11 View

What's the potential of metal enclosure of an LED luminaire not connected to ground with a Class 2 driver for providing input?

I have a 13 W, 24 VDC luminaire, getting input from a class 2 driver. I had a debate about whether dielectric test is required on the luminaire if the driver is CLass 2, UL listed. And, while I...

09 September 2018 5,894 2 View

Are Direct Markets Better Options For Selling Agriculture Goods By Farmers?

Are Direct Markets Better Options For Selling Agriculture Goods By Farmers?

13 May 2018 1,184 13 View

For a 3*4*2 tensor 'y' how to find a matricization form of order 6*4 i.e. Y of({1,3}*{2}; 3*4*2) ?

01 January 1970 1,984 3 View

Http://epubs.siam.org/doi/pdf/10.1137/07070111X In this pdf at page 458 figures are given, I need to know in which software I can draw??

I need to explain 3-way tensors by using these diagram and also I want to know that how to draw them and in which software?

01 January 1970 2,448 3 View

Is there any literature review on child immunization in India with machine learning algorithms?

I am currently working on the immunization dataset of India and not able to find research papers for literature review regarding my field. Suggest if there is any work.

01 January 1970 4,937 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View