Since they are also made up of nucleotide units (DNA) so how a little change in temperature or other conditions eliminate the suicide vector but the same condition has no effect on the genomic DNA or other vector present in the same host?
I have a .text file for various FASTA sequence , and i want to convert these sequences into a numeric file which will be in .csv format. OR I want to extract physiochemical properties(features)...
25 July 2024 3,650 2 View
I am trying to replicate results published in the journal paper published recently (Modeling of Laser-assisted micro-milling Inconel718 ). In that paper, they used vdflux subroutine to obtain...
05 July 2024 3,701 0 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
As the biaxial loading may implement additional hardening of the material during fatigue with an increase in the biaxiality ratio. What would be the effect of planer biaxiality on dynamic strain...
31 May 2024 9,104 0 View
Ethics
15 April 2024 6,778 1 View
Hello! I want to know how to test the electrochemical stability of the electrolyte (2M ZnSO4 solution) I have measured electrochemical stability window of a electrolyte by 3 electrode...
26 March 2024 3,306 1 View
The reason I suspect that the beat note should not be broad linewidth (~100 MHz), as I see in the spectrum analyzer, is because, using those same lasers, we can create a magneto-optical trap....
17 March 2024 3,195 4 View
LAH is probably out of the question so maybe H2/Pd could work. But the nitrile would be stabilized due to resonance from the aromatic ring so I do not know if this will work. Of course, we could...
04 March 2024 9,983 1 View
I would like to know the void fraction for known powder material properties to estimate a bisque sample's packing density/void fraction. In combination with any geometric &/or Archimedes...
12 February 2024 9,592 1 View
Dear all, Will you please let me know how to calculate the carbon, nitrogen, and phosphorus (C: N:P) ratio in wastewater? Is it advisable to measure total COD, nitrogen, and phosphate? or I...
05 February 2024 4,105 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View