Satya Deo Pandey

4 Questions 6 Answers 0 Followers

Questions related from Satya Deo Pandey

What does it mean if you say 20-25 OD A254?

I read an article where they said, they used 20-25 OD A254 sample for a ribosome separation on sucrose gradient. I know this is an important parameter but I am not getting it how much does that...

20 February 2019 3,711 1 View

Why does aztreonam ineffective against gram-positive bacteria, Is the permeability a reason or poor affinity towards PBP3 of Gram +ive bacteria?

Can anyone explain, Why does aztreonam ineffective against gram-positive bacteria, Is the permeability a reason or poor affinity towards PBP3 of Gram +ive bacteria? please explain.

01 June 2017 6,528 3 View

Lipid profile of a complemented strain is matching with mutant strain, not the WT strain but the physiological features like biofilm are same as WT?

In one of my experiment with wild type (WT), mutant strain and complemented strain (CS) of M. smegmatis, glycopeptidolipid (GPL) profile of mutant and CS are exactly matching, leaving a single...

19 September 2015 512 4 View

Why do I get PCR amplification but no release of the fragment when digested with appropriate restriction enzymes?

Has anyone come across a situation when you get a amplified band in PCR, using a recombinant vector as template (tested alongside the positive control; genomic DNA as template) but no release of...

21 July 2015 7,189 4 View