Satya Deo Pandey

6 Questions 7 Answers 0 Followers

Questions related from Satya Deo Pandey

What does it mean if you say 20-25 OD A254?

I read an article where they said, they used 20-25 OD A254 sample for a ribosome separation on sucrose gradient. I know this is an important parameter but I am not getting it how much does that...

20 February 2019 3,750 1 View

How does the suicide vector get eliminated from a recipient cell by changing the temperature or some other conditions?

Since they are also made up of nucleotide units (DNA) so how a little change in temperature or other conditions eliminate the suicide vector but the same condition has no effect on the genomic DNA...

28 October 2017 6,182 0 View

Why does aztreonam ineffective against gram-positive bacteria, Is the permeability a reason or poor affinity towards PBP3 of Gram +ive bacteria?

Can anyone explain, Why does aztreonam ineffective against gram-positive bacteria, Is the permeability a reason or poor affinity towards PBP3 of Gram +ive bacteria? please explain.

01 June 2017 6,578 3 View

Lipid profile of a complemented strain is matching with mutant strain, not the WT strain but the physiological features like biofilm are same as WT?

In one of my experiment with wild type (WT), mutant strain and complemented strain (CS) of M. smegmatis, glycopeptidolipid (GPL) profile of mutant and CS are exactly matching, leaving a single...

19 September 2015 552 4 View

Why are electro competent cells (prepared in 10% glycerol) of mycobacteria not transformed upon storage into -80C? Do they die, due to cold shock?

I had prepared electro competent cells (in 10% glycerol) of Mycobacterium smegmatis to inactivate a gene using electroporation a mode of transformation and homologous recombination a method for...

28 July 2015 5,198 3 View

Why do I get PCR amplification but no release of the fragment when digested with appropriate restriction enzymes?

Has anyone come across a situation when you get a amplified band in PCR, using a recombinant vector as template (tested alongside the positive control; genomic DNA as template) but no release of...

21 July 2015 7,233 4 View