How does the process of gene cloning work and what are the steps involved in cloning a gene?

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Difficulty in cloning of 1kb gene into vector?

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RNA-seq DEGs downstream analysis and reporting in literature?

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Theresa Smith

Are you referring to cloning a gene as in inserting a gene into a new DNA plasmid or vector?

Vineetha Kudala

DNA cloning is a molecular biology technique that makes many identical copies of a piece of DNA, such as a gene. In a typical cloning experiment, a target gene is inserted into a circular piece of DNA called a plasmid Hint: The insertion of a gene into the plasmid is known as gene cloning, which takes place to insert a specific gene into the plasmid vector for its transformation.