I incubate the soil with earthworm and applied different concentrations of NMs. My pH data looks strange to me. Previously, literature documented that earthworms improve soil pH. Does anyone conduct the experiment to evaluate the pH response over combine exposure of earthworm and NMs? My weekly data is changing in a dose-dependent manner. pH should be increased or decreased with time and dose?

Please give me a clue regarding this issue. Your response is highly appreciated.

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