What kind of connectors and how to connect it to my small patch antenna?

I have designed a microstrip patch antenna that has a dimension of 13 mm X 8 mm. The microstrip line width is around 500 micrometers. The substrate is not PCB or FR4, for some reason, I can not...

10 February 2020 7,478 6 View

Purification of Octapeptin C4?

I am doing synthesis of Octapeptin C4 analogues with variable tail moieties as part of my project. I have made the final compound, which is cyclized and all BOCs removed. The problem is, I can't...

16 December 2019 1,730 2 View

What is best method for biofilm inhibition assay?

04 September 2017 7,142 3 View

How to measure galvanostatic charge discharge of supercapacitors?

I am trying to measure galvanostatic charge discharge of supercapacitors by electrochemical workstation. It has three options to measure GCD; based on potential/ charging time or both. Which one...

09 February 2017 3,043 4 View

Does the capacity of Oxygen to donate lp electrons increase with temperature? If it does, what is the transition temp where it increases notably?

Mainly we're investigating the effect of temp on released oxygen during hydrothermal reduction of GO

15 January 2017 5,202 1 View

What are the procedures of lean burn of biodiesels in a diesel engine?

I want to achieve low temperature combustion by biodiesels

05 March 2013 9,117 1 View

Define peer review journal?

Many journals without having an expertise memebrs in Editorial board and just after publishing a single volume claiming as Peer-review journal.

01 January 1970 2,427 15 View

Myths regarding child vaccinations; a global concern.

How are parents viewing child vaccination in your region? The misconception regarding vaccinations is something to be addressed. How are doctors and health care system is addressing this issue...

01 January 1970 5,468 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View