You have to use a "soft" way for the ionisation of the analyte. In my opinion, ESI TOF-MS would be the best way. But than you do not need to seperate the analyte by IC?
thank you oliver..but i have some clarification in this part..Tanin have different subunit. with the help of microbe it form tannase for the production of gallic acid..how know that substrate fully converted to product...