I want to quantify UREA in cell culture media. I read about diacetyl monoxime method and Jung reagent method. Jung reagent is costly and diacetyl monoxime method needs boiling temperature. Is there any other simple method?
You can measure urease activity (paper of Tabatabai and Bremner is attached)
You can measure urease activity (paper of Tabatabai and Bremner is attached).
how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.
23 July 2024 4,649 2 View
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
18 July 2024 2,420 0 View
Kindly confirm can we do MTT assay for quantification of apoptosis. Is MTT Assay a valid assay to quantify apoptotic cells ? What about LDH assay ?..how is it better for apoptosis quantification...
08 July 2024 5,482 2 View
We want to purchase few units
26 June 2024 3,068 0 View
I needed help to get the published final version of a research article titled: "Alterations in placental long chain polyunsaturated fatty acid metabolism in human intrauterine growth...
11 June 2024 3,120 2 View
I am trying to measure telomere length in human DNA samples through qPCR. In the protocol, they have not included extension step. Can anybody explain how a qPCR can work without an extension step?...
31 May 2024 514 2 View
Im looking for the Cloud Technology research guide in mangalore. Can anyone recommend some names on which we discuss ?
28 May 2024 2,865 1 View
Quality Infrastructure ( QI ) has been established across the globe to create a mechanism for independent third party assessment of products, services and processes. It plays a pivotal role at the...
16 May 2024 4,703 10 View
Satellite images are available for various bandwidths. How can we reap benefits of AI to classify and recognise the status of water bodies? How can we predict the availability of quality water for...
16 May 2024 3,523 2 View
There are numerous cooking oils are available in international markets for mass consumption at an affordable prices. Due to health benefits, can we make available the Olive Oil at reasonable...
08 May 2024 4,526 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...
06 August 2024 1,350 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...
01 August 2024 9,349 2 View
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View