How does one perform Insulin and Insulin like growth factor assays?

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Is it necessary to autoclave every vessel used in DNA isolation?

DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?

10 July 2024 7,526 3 View

What are the common mistakes occur during DNA isolation in plants?

General mistakes which could happen during DNA isolation of plants

10 July 2024 9,488 3 View

If I have 1000 or more plant extracts, which is the best latest technology for the rapid and highly accurate identification of secondary metabolites?

the query centers on determining the most efficient and precise latest technology available in the market, capable of swiftly analyzing complex mixtures while maintaining a high level of...

26 May 2024 2,898 6 View

State wise impact of soil health card scheme for fertilizers consumption at farm level in India ?

impact scenario of fertilizers consumption pre and post scheme of soil heath card

06 May 2024 5,219 4 View

We found a Raman peak in the silver powder around 1000 cm-1. What could be the origin of this peak?

As we know, for Raman spectroscopy, a change in dielectric constant is required w.r.t. phonons, which does not apply to metals. Therefore, pure metals are Raman inactive. Also, this 1000 cm-1 peak...

02 March 2024 1,856 4 View

What are the major difference between the Industry 4.0 and Industry 5.0?

In industry 4.0, major focus was on circular economy with automation.

30 January 2024 6,722 2 View

Sweetest variety of strawberry is..............?

Which is the most sweetest variety of strawberry in Indian condition.

06 January 2024 1,839 1 View

Which is the best cultivar for meadow orcharding in guava ?

Best suited guava cultivar for meadow orcharding in India.

06 January 2024 5,496 1 View

Please explain this mango fruit ?

Two stones mango fruit called as..............

06 January 2024 2,276 3 View

how to analysis the water foot print of farm house hold in India ?

26 September 2023 7,116 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

Is it true that science is leaving the era of mathematics and entering the era of matrix mechanics?

We assume this to be true. Science leaves the era of mathematics and enters the era of matrix mechanics and the turning point is the discovery of numerical statistical theory called Cairo...

31 July 2024 3,900 2 View

How can you draw a coordinate bond between a metal and donor atom in metal complexes, using discovery studio visulizer software?

Hello,, The metal complex ligand appears incomplete in the screen of the discovery studio visualizer

30 July 2024 3,354 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View