How does one perform Insulin and Insulin like growth factor assays?

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How Nickel affect biological methane production ?

Soil fertility, plant nutrition

23 December 2020 7,301 5 View

What are the criteria one must look into while searching for a research guide?

As we know that guide plays a vital role in the entire tenure of research leading to PhD, what are the qualities a scholar must look into consideration while selecting a guide?

19 September 2020 2,538 18 View

Mechanism of DNA replication?

I have read this in a book that DNA polymerase can discriminate between ribo and deoxyribonucleoside triphosphate bcoz of steric exclusion of rNTPs from the DNA polymerase active site, as the...

08 June 2020 2,262 6 View

Key elements to consider before rolling out the final survey?

hi, for my quantitative research, i am analysing the pilot survey data. While, my cronbach is looking good, but Tstat, few outer loadings & Pval doesnt look good. Which are the key...

01 May 2020 6,426 10 View

In biosorption process (Lantana camara leaves as biosorbent), why do we get a precipitate after putting the filtrate on hot plate for agitation?

Usually, we get a clear filtrate after performing batch experiments; same is with Lantana indica leaves as biosorbent. But, after filtration we observe dark precipitation in this case. Is it due...

03 April 2020 9,406 1 View

Suggest me the literature related ternary 1D Photonic crystal using TMM method and Matlab Code?

Ternary 1D Photonic crystal using the TMM method and Matlab Code.

17 March 2020 5,970 3 View

Who decides iLref (ref current) for a closed Loop control of Grid Connected inverter?

Power (active, reactive) send to grid depends on iL magnitude and its phase wrt grid voltage. For a closed-loop current control of a VSI (as of now let's consider a single-phase VSI) in a...

14 March 2020 1,810 4 View

What is the best measure of productivity of the faculty/researchers?

There are good number of measures are available to measure the research productivity of the faculty members/scientist like, 1.Scopus "h" Index of the author 2.Google Scholar "h' index of the...

20 February 2020 631 3 View

Which one is best measure of publication quality- SJR or Impact factor of the Journal ?

Some research journals having Impact factor and some having SJR. Which one is the best measure of the quality of reserach paper?

11 February 2020 6,497 3 View

What is G index?

Generally we talk about the h-index of the author or journal. What is G index? is it similar to h-index?, means the productivity measure of the author/researchers. If the author having h-index...

15 January 2020 5,256 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View