in vitro cytotoxicity study - SRB assay
High Growth Inhibition (GI50) value almost similar in tested cancer cell lines and the HEK 293 and 3T3L1 (normal cell lines).
What does it say?
Also, can i use the extract for in vivo studies?
Should and will the extract show the same result?
HI,
if one of us could predict, that an in vitro efficient drug will work in the same manner in vivo, we would earn a lot of money in a pharmacological focussed company. There are way to many additional aspects you have to see ( liver metabolism, multicellular system...). Further more the use in animals can also be limited by factors like detergent or diluent of your compund, how to administer... Try it first with primary cell models and think if your concentration is somehow realistic ( you can not ad 5% of body weight in form of DMSo to a mouse), and be sure your controls were absolutely fitting
regards
Sven
Understood Sir. And yes, seeing to all the criteria you have mentioned. Thank you very much.
In addition to Sven's rsponse, if your extract displays similar "growth inhibition" in normal and cancer cells, the probability is high that you extract contains toxic substances.
The possibility remains that there are not the same compounds in your extracts that kill the normal and the cancer cells.
An extract is of limited value to actually treat cancer patients, and therefore to initiate costly and time-consuming in vivo experiments.
The best way to proceed is to go to bioguide fractionation of your extract on two cancer and two normal cell lines and see what will be the compound(s) you will isolate at the end as those responsible for growth inhibition.
Best regards
Robert
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line