How does one extract microbial DNA from human tissue without contamination from host DNA?

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Cornelia Virus

Hi, there are some old discussions here on researchgate covering the same question:

https://www.researchgate.net/post/How_to_separate_microbial_cells_from_human_cells

https://www.researchgate.net/post/How_can_I_differentiate_extracted_bacterial_DNA_from_animal_DNA

Also this article might be helpful:

Anaerobe. 2010 Feb;16(1):47-53. doi: 10.1016/j.anaerobe.2009.04.009. Epub 2009 May 20.

New methods for selective isolation of bacterial DNA from human clinical specimens.

Horz HP1, Scheer S, Vianna ME, Conrads G.

Parise Adadi

Article Microbial DNA extraction from intestinal biopsies is improve...

Article Optimal DNA Isolation Method for Detection of Bacteria in Cl...

http://www.scielo.br/pdf/cr/v46n7/1678-4596-cr-46-07-01223.pdf

Ali Akgul

I think you might use a filter to only get bacteria. I used that for RNA work. You would choose the filter size for separation based on the cell you work on and bacteria you focus on. After that step I believe that using any kit for DNA should work.

Good luck,

Ali