I have two images in which the first one shows the presence of bacteria on a plate seeded with macrophage and yeast for the purpose of phagocytosis assay. The second image shows the presence of something arranged in a very uniform and dense pattern in chains which diverge from one another and take the shape of a leaf. I have one thought which is that these bacteria have outgrown the yeast and macrophage in the first. In addition, macrophage were unable to take up huge number of cells growing in the plate. Moreover, when the media components were about to exhaust, these bacteria which are yet present while yeast and macrophage bursted, have arranged themselves in these chain like forms. Please give your opinion if someone has come across this or agrees with me or have some different thoughts on the same.
Hello,
What do you mean by "when the media components were about to exhaust..."?
Is your sample dry? Because for me it looks like crystals (leaf, branching).
Yes, I took out the media and let it dry, meanwhile I took the images. But no dye was added. No substance was added which may crystallize. Actually, by media components exhaust, I mean that after sometime when bacteria are in huge number and the media is in the form of a layer, when the bacteria are not able to move anymore in the drying layer or due to exhaustion, they are not able to reproduce anymore.
Kaushiki Chatterjee I had seen it all on the petri dish. To the extremities, this pattern was visible. May be you are right. But I am trying to find whether anyone has seen bacteria getting arranged like this. I want to add to the discussion that this pattern was visible at or after approx. 12 hrs of seeding. I seeded the cells at 3 pm and the murine peritoneal macrophages were collected by pipetting the cells collected in syringe to falcon tube in the open. I doubt that even a single bacteria existing will be able to make over 2^36 cells after 12 hrs so that they outnumber the other cells. Further, not even a single cell is visible ( macrophage or yeast). The second image contaminating the macrophage was taken at 9 pm.
I took these images of the same brand of Petri dish I used earlier. Please have a look. As suggested by Nawel Zaatout I think that she is indeed right.
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