Dear  Friend,

The answaer link to your question that  there are two types of mutation take place in plant  cell at  micro level and maro level.  In  some cases, the shift mutation take place  which is linked to  an  any  important characteristics.   There are many genes involve  during  development of mutant  through chemical or physical mutagen.  The knowk out gene  of specific is not possible. If you have analysed  whole genome after  and before mutation , then only  you can  able to get few idea for further research.

Dear  Friend,

The answaer link to your question that  there are two types of mutation take place in plant  cell at  micro level and maro level.  In  some cases, the shift mutation take place  which is linked to  an  any  important characteristics.   There are many genes involve  during  development of mutant  through chemical or physical mutagen.  The knowk out gene  of specific is not possible. If you have analysed  whole genome after  and before mutation , then only  you can  able to get few idea for further research.

Dear Maren:

Due to the proper nature of the mutations induced by X-ray, I think that you must to analyse a big number of putative mutants to find the specific mutation that you are looking for. Because, when you are applying the X-ray treatment this will be affecting the whole genome and you can observe multiple mutations, could be included the one for the gene you expected to see. In conclusion, the ratio in which you will observe one specific mutation will be around 1 in 10000 to 100000. Also you must to take into consideration the inheritance of the gene, if it is single o multi-genes coding for a qualitative or quantitative character; in some case for single gene inheritance is possible to get the favorable mutation in 1 to 1000. I wish you the best in your future work,