How to analyze EEG data from Smarting (xdf) with Fieldtrip?

Dear all, I would like to analyze my Smarting EEG data (xdf) with Fieldtrip. Has anyone ever done that and can give me some tips? Unfortunately I have not found a function for the import of...

18 November 2019 2,403 3 View

How to predict the crack lengths of aluminium lap joint subjected onto different amplitude loading?

how to predict the crack lengths of aluminium lap joint subjected onto different amplitude loading?

02 May 2019 7,970 2 View

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We isolated LDL from human plasma by ultracentrifugation followed by dialysis (we changed from PBS to NaCl) overnight. Afterwards we oxidized native LDL with CuSO4 for 24 hrs but, after oxidation...

15 April 2014 5,399 3 View

Can someone help me regarding cross reactivity in detection of immune precipitation?

I precipitated my protein with an antibody generated in a rabbit. For detection I use one that is generated in a mouse. When detecting I still observe the heavy and light chain. I guess this is...

17 June 2013 6,998 3 View

Can I use a polyclonal antibody for quantification?

I want to quantify eGFP in cell lysate via Western Blot. Are there any disadvantages of polyclonal antibodies in comparison to monoclonal antibodies, besides possible cross reactions?

13 November 2012 3,247 3 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How to convert isotrophic Deriche filter into anistrophic?

12 February 2021 5,889 6 View