Hi everyone,
I am looking for a commercial antibody that detects the C-terminal region of ApoB100 to differentiate between ApoB48 and ApoB100. Any ideas, vendor suggestions are highly appreciated! Thanks!
Hey guys, do you know the solubility of SAC in DMSO? I need SAC solution for my SPR binding assay and can't find the fitting molarity. Only hint I got was 10 mM but I tried it yesterday and it...
31 July 2023 842 0 View
Dear all, we are currently having trouble with our Westernblots. Attached you can find two pictures of our blots. We use fluorescently labelled antibodies, but even if we do not incubate the...
19 June 2023 7,660 3 View
Hello, I'm working with H9 derived OPCs (human) (Merck). We used to detect the myelination with the antibody AB980 from Merck Milipore. Unfortunatly they took it out of range. We tried the...
17 December 2019 7,006 0 View
Dear all, I would like to analyze my Smarting EEG data (xdf) with Fieldtrip. Has anyone ever done that and can give me some tips? Unfortunately I have not found a function for the import of...
18 November 2019 2,698 3 View
how to predict the crack lengths of aluminium lap joint subjected onto different amplitude loading?
02 May 2019 8,135 2 View
I have some AKSS data that I want to analyse, but I cannot find the scoring manual for this questionnaire. The questions should be weighted differently but I cannot find information on how to do...
14 October 2018 2,573 2 View
This question concerns the correlation coefficients reported by AMOS for latent constructs. These differ from the correlations obtained for the composite constructs by, for example, SPSS. However,...
27 March 2018 4,708 3 View
We isolated LDL from human plasma by ultracentrifugation followed by dialysis (we changed from PBS to NaCl) overnight. Afterwards we oxidized native LDL with CuSO4 for 24 hrs but, after oxidation...
15 April 2014 5,532 3 View
I precipitated my protein with an antibody generated in a rabbit. For detection I use one that is generated in a mouse. When detecting I still observe the heavy and light chain. I guess this is...
17 June 2013 7,116 3 View
I want to quantify eGFP in cell lysate via Western Blot. Are there any disadvantages of polyclonal antibodies in comparison to monoclonal antibodies, besides possible cross reactions?
13 November 2012 3,399 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hi, I'm curious to know if data on chemical compounds from PubChem, such as water solubility properties, can be used to train a machine learning model for commercial purposes. Will this infringe...
30 July 2024 8,707 1 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View
I am working on flourescence sensing of heavy metals using quantum dots.Can any of you suggest how to find the detection limit. Whether it is calculated from formula or we can get from instrument?
19 July 2024 7,411 3 View
Dear Participants- Faculty and Researchers from Pakistani Higher Education Institutions, Thank you for participating in research study that aims to 'Analyze Individual and Institutional Factors...
18 July 2024 7,024 1 View