Unfortunately i get a lot f patients who inadvertently takes steroid for years and then presents with 20 ACI . What rate of decrement do you practice?
Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...
22 February 2021 3,680 4 View
the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please
16 February 2021 8,801 3 View
There are a few papers dealing with the effects of climate change on the pollination of European orchids, but I wonder if similar studies have been carried out in the Tropics.
04 February 2021 1,818 4 View
Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...
04 February 2021 8,993 2 View
Dear researcher, After amplifying my target gene (1900bp) into the required cDNA by PCR,>cut specific gel bands and purify the gel and the concentration was 30ng/ul. Next, performed A-Tailing...
21 January 2021 2,671 3 View
Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...
20 January 2021 9,019 1 View
Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...
20 January 2021 5,557 2 View
I need to simulate a mushroom FSS and I'm struggling with the ports. What's the correct way to define them? Should I construct a proper SMA connectors, or is it enough to put a waveguide ports...
19 January 2021 6,590 4 View
Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...
15 January 2021 2,293 3 View
Dear Researchers, While working on plant gene transformation, so after cDNA + Gene Primer amplification, >the PCR/Gel were purified, so next for A-tailing and TA cloning is mandatory to...
12 January 2021 3,272 34 View
hi, I'm going to determine the dosage of testosterone in order to inject to rats or mice subcutaneously. I wonder that is there a particular dose or multiple doses for that or not. thanks.
05 January 2021 5,921 2 View
I work with a steroidal ketone and ethylene gylcol (EG). I would like to prove that ethylene glycol forms a hydrogen bond with the carbonyl group of the steroid. The problem is that so far I have...
26 November 2020 2,814 3 View
Suppose, one is doing enzyme assay of an enzyme of a steroidogenic pathway. Is it necessary to deplete the steroids from the tissue/ cell homogenate before administering substrates to check enzyme...
21 September 2020 7,439 2 View
Our plant sample contains steroids based on the phytochemical results, and were having a hard time looking for some references about steroids' effect on lowering blood glucose level on mice.
01 February 2020 4,740 3 View
31 January 2020 9,763 3 View
I am attempting to recreate an extraction of steroidal glycosides from Lily bulbs from this paper Isolation and Structural Determination of Steroidal Glycosid... To do so I need to run GPC on a...
28 January 2020 9,047 2 View
Hi all, is it possible to extract RNA and steroid hormones from the same tissue? My current strategy is to macerate the animal tissue sample and divide into equal portions, one for TRIzol RNA...
28 July 2019 1,579 3 View
I ran 2 cell proliferation assay on cardiac fibroblasts under varying treatment conditions (cortisol 0.5uM, Cortisol1uM) (TGF-b1, TGFb1 + Cortisol 1uM, TGF-b1 + SB431542) the results suggest that...
06 July 2019 1,288 4 View
06 July 2019 561 3 View
solvent system energy other parameter
05 June 2019 8,046 1 View