I want to measure average size of my nanoparticles in SEM picture by imageJ and find their distribution.
I think threshold section play an important role ,because I change it ,my average size have various changes. How can I adjust threshold section? Is there a particular rule in this section?
for distribution of grain size, I used distribution and histogram section, but I can't draw distribution of grain size. It calculates distribution of area or mean of grains.
I send my SEM picture. please tell me about average size and distribution of grains size.