Dear Nachiket

Thanks for your response.

I want to measure average size of my nanoparticles in SEM picture by imageJ and find their distribution.

I think threshold section play an important role ,because I change it ,my average size have various changes. How can I adjust threshold section? Is there a particular rule in this section?

for distribution of grain size, I used distribution and histogram section, but I can't draw distribution of grain size. It calculates distribution of area or mean of grains.

I send my SEM picture. please tell me about average size and distribution of grains size.

Thanks for your attention.

Dear Sir,

You can use ShapeLogic Plugin (http://www.shapelogic.org/particle.html). I hope to help you.

Best wishes,

Ion C. Andronache.

Hello Mam,

If u want use Image J to estimate Grain size

--> open the pic.

--> go to line in the Tall box

--> draw line matching withe the scale line

--> go to analyse and open (set scale )

--> put the number of pic's scale in the third space

--> change the unite to nm or any and then go to pic. and start yr work by drawing line matching with grain ead.

by CTRI+M bot. u can gate table for your data and u can do a lot analyzing by image J