Dear colleagues,
I am trying to treat 55.1c cell lines with palmitic acid which came as a powder.
I found a protocol saying I should incubate palmitic acid with 0.01 N NaOH for 30 mins and complex it with 5% BSA afterwards. This results in a 20 mM solution of palmitic acid which can then be mixed with cell medium.
But mixing it with medium/reaching room temperature makes the palmitic acid turn back into it's solid form floating on the medium...
Has someone experienced this as well?
Is there anything I forgot to do?
Thanks in advance!
Steve Duo
Dear Steve t. The powder generally represents palmitic acid in sodium salt which is not the best option but can work. However, you can still make your mixture. Incubate the desired amount of palmitic acid with a solution BSA fatty acid free, very important, up to 32 mg/ml in PBS 37 degrees C for 30 minutes in constant mixture, avoid buble formation. You can calculate the amount you require in your media, the amount of BSA (32 mg is the concentration in the plasma) and the number of palmitic acid since you can bind between 5 and up to 9 molecules of palmitic acid per BSA. regards,
Dear Steve t. The powder generally represents palmitic acid in sodium salt which is not the best option but can work. However, you can still make your mixture. Incubate the desired amount of palmitic acid with a solution BSA fatty acid free, very important, up to 32 mg/ml in PBS 37 degrees C for 30 minutes in constant mixture, avoid buble formation. You can calculate the amount you require in your media, the amount of BSA (32 mg is the concentration in the plasma) and the number of palmitic acid since you can bind between 5 and up to 9 molecules of palmitic acid per BSA. regards,
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