I am in the middle of a study on circulating microRNAs profiling, but I would love to combine this study with another one. As I am storing serum in liquid nitrogen for further microRNA analysis I was wondering to do the same thing with CTCs. My problem is how do you preserve, collect or archive blood samples so you can study CTCs later on. How long can you archive these samples and in what temperature? (I do not want to do it in real time and most importantly do not have funds for it right now)
the fresh samples is the best one because CTCs are really sensitive and rare and you may miss your cells if you keep them along...
you can collect CTCs from whole blood with MACs or Easysep assay manually after that you should keep them in fortify media but no article explain about the media and i think it is in trial
Well, we did a long time and have a medium database as well as frozen CTCs but noone ever asked for... Now we concentrate on real time monitoring, which is much more effective.
What was your goal in preserving and archiving?
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