We have an adipoqCreER rosa26Tdtomato mouse that we can see the adipocytes under flurescence microscopy but we need to isolate these tomato+ cells so that we can analyze or sort them for qPCR etc. by FACS.
Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.
26 October 2020 708 2 View
I am doing an optimization of ginger extract using doe software, firstly I extract the ground ginger with 20ml solvents of 60%, 70% and 80% ethanol using the ultrasound water bath in different...
23 October 2020 3,268 10 View
Has anyone tried using addgene LentiCrisprV2 (Plasmid # 52961) to knockout a mouse gene? The cells were resistant to puro after infection, however, none of my gRNAs can knockout the gene.
19 May 2020 1,180 3 View
I want to design some concrete mixes with different fly ash/binder ratios (can be as high as 80%) but the same strength level. The water/binder ratios (together with superplasticizer dosages) have...
12 May 2020 8,551 6 View
Hi, I am genotyping newly constructed trangenic mice knocking down target gene. I have got F1 generation from F0. And DNA examination indicates that the target DNA fragment has been excised. The...
27 April 2020 5,450 3 View
According to the color or the loading buffer and the protein ladder, the pull-down progressed successfully. And I also get right signals of beta-actin (lower ones), but another primary antibody...
28 August 2019 3,702 7 View
Hi everyone, I am conducting an experiment to detect copy number variation among my gDNA samples using RT-PCR. I have generated standard curve for the genes of my interests and two controls...
23 July 2019 6,930 3 View
Dear fellow scientists, I'm looking for a fixable viability dye for flow cytometry. Because I'm doing a time sensitive T cell stimulation assay, I need to add the viability dye before starting the...
09 July 2019 4,877 4 View
Hi, everyone, I would like to quantify the Abeta load of my IHC staining images. How should I do it? someone use plaque area %, someone use plaque numbers, which one is better? And can I do it...
15 February 2019 2,109 1 View
There are lots of literature in the language testing and assessment field. I am wondering which one in your own perspective inspires you the most, or helps you in a ways that is really...
17 November 2018 947 5 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...
03 March 2021 961 3 View
Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...
02 March 2021 1,757 4 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.
02 March 2021 1,516 4 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...
28 February 2021 8,189 3 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View