How do you handle the organic solutions of lipids in small volumes while preparing liposomes?

20 January 2015 1 2K Report

It is possible that the micropipette tips/eppendorf tubes/plastic vials may interact with the chloroform solution of lipids like PC which may cause stability issues? How do you transfer or handle such solutions in the microliter range while preparing liposomes?

Robert Anthony Roggers

I agree with Francois. During the course of my lipid work I used graduated glass pipettes for transferring lipid solutions. You can generally find them from any glassware supplier and, at least from Fisher Scientific, are available in volumes from 100 mL to 0.1 mL.

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