This review article would give you enough information about available measurement techniques:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4292845/

You can see several methods for absolute telomere length measurement by qPCR in these articles:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3047434/

https://www.ncbi.nlm.nih.gov/pubmed/18476834

You should be careful when using qPCR - PCR-based techniques (similarly to TRF technique) require high-quality DNA that is not compromised by degradation. In fact the available hybridization techniques are currently considered as a gold standard.

Well known and already proven commercial kits are:

1. TeloTAGGG™ Telomere Length Assay

https://www.sigmaaldrich.com/catalog/product/roche/12209136001?lang=en®ion=BG

2. TRAP Assay

http://www.merckmillipore.com/INTL/en/product/TRAPeze-Telomerase-Detection-Kit,MM_NF-S7700?ReferrerURL=https%3A%2F%2Fwww.google.bg%2F&bd=1

YOU CAN USE THIS ARTICLE

Please download Telomere length measurement. by quantitative real time PCR

We did this recently:

Article The differential spatiotemporal expression pattern of shelte...

In contrast to hybridisation techniques, you need much less starting material for the qPCR.

Hello

Kindly look at this file will help you

The best method would be Q PCR due to its high through put,fastness and needs few starting material. i do have also aplan to Telomere length measurement study i saw cawthon and o callaghan studies but still i need more clarification how to do it and how to analyze the data after a PCR. any one have any ideas please let me know it would be really appreciated.