Dear all, Can any one say how to calculate convective mass transfer coefficient and evaporative heat transfer coefficient in fluent?

Is it possible to find in fluent? Any other method is there?

10 September 2020 5,492 3 View

Why charges should induce when Glass rod is rubbed with silk cloth?

Here I mentioned only Glass rod and silk cloth. This can be anything from triboelectric series. But, why should it happen? Why should different charges accumulate in two materials and then...

22 August 2018 2,619 2 View

Two-dimensional approximation of cylindrical bar cropping process - plane stress or plane strain?

19 July 2017 1,236 3 View

Can someone explain the steps for calculating the electron transfer number in oxygen reduction reaction?

When the ORR catalyst i synthesized gives five times greater current density than the commercial Pt/C, then the slope of the KL plot for the catalyst synthesized will be higher because of the...

24 July 2016 1,504 2 View

Are you familiar with Cp values for 18S rRNA?

14 April 2015 7,664 28 View

Why or how does serum reduce the transfection efficiency?

24 March 2015 9,996 6 View

Why do most vectors have 2 origins?

I am using two different systems for my RNAi screen and both of them seem to have 2 origins of replication and I am not clear what the reason is? One of them has a f1 and pUC ori and the other has...

06 March 2015 2,685 6 View

How can I integrate FORGE (Bulk forming results) into ANSYS for structural validation considering pre-stress?

To import mesh with pre-stress values into ANSYS?

26 January 2015 671 3 View

Is there a network for people researching on phase change materials PCM? or design of any type of heat storage?

I am presently researching on PCMs. I wonder if there is a network or a platform for people researching on energy storage/PCMs or on similar fields. Thank you Regards

22 January 2015 10,004 6 View

Is there any particular reason for using HEK cells for lentiviral packaging?

Hi all, dor lentiviral packaging, I used both HeLa and HEK cells and I found out that packaging had occurred in HEK but not in HeLa. Is there any particular reason for that? Has anyone tried...

20 November 2014 5,279 7 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View