How do you dissociate a 40 micron thick PFA fixed mouse brain slice?

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Alpana Singh

Hi,

I would suggest dissociation of your brain slice into single cell suspension before PFA fixation. There are many protocols available online, you can go through them.

Thanks,

Alpana

Steingrimur Stefansson

Hi Leslie Spitalny . If your fluorescent protein is intracellular, you could try freshly made 0.25% trypsin, 0.25% proteinase K in TBS.