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Questions related from Leslie Spitalny
I would like to use flow cytometry to analyze single cell expression of fluorescent expressed proteins. How do I dissociate a 40 micron thick, 24 hour PFA fixed mouse brain slice to achieve a...
11 August 2021 8,575 2 View
I am trying to lift, dissociate, and then flow fixed HeLa cells for fluorescence analysis. When doing this previously, the cells lift from the plate with 0.5% trypsin-EDTA treatment but they then...
30 July 2021 8,840 4 View
I am fixing HCT116 cells to use with a photo activation probe for cell membrane labeling. I have used 1% and 4% PFA in water as a fixative and fix on ice for 20 minutes. I then quench the reactive...
30 July 2021 4,816 0 View
I am using confocal microscopy for photo-probe activation which requires photobleaching. The photobleaching rate of the fluorophores used for imaging will dictate the time of sample exposure. How...
16 June 2021 8,627 0 View
Utilizing a aminoallyl-oligo of 33 base and a C12 linker, we are conjugating succinimidyl ester fluorophores together. After HPLC purification, what is the best way to quantify reaction yield and...
03 June 2021 2,547 3 View
I am trying to measure the absorption spectrum of a photo-caged fluorescent probe on an Agilent Cary 100 UV-Vis. I tried a 10mM solution in DMSO after a DMSO blank and the spectra had a broad flat...
11 March 2021 9,979 5 View
I am using the scratch/wound healing assay as a platform to study cancer metastasis. This assay should mimic cancer cell migration for metastatic tumor initiation. However, after reading many...
27 August 2020 715 4 View