This is for a current research on insilico construct of a multi-epitope subunit vaccine
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View
I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.
24 February 2021 1,633 4 View
I am new researcher. I want to learn more and I need your help. Thank you.
04 February 2021 6,834 4 View
I am currently looking to transfect HeLa cells using lentivirus and Lipofectamine 2000 and have been looking for cited protocols. Would anyone have any that they are willing to share?
31 October 2020 5,639 2 View
Greetings, I'm doing a screening design and want to allow for lack of fit. I need replications so I want to add four additional points to my dataset. Rather than use center runs I use corner...
11 October 2020 9,672 1 View
The antimicrobial activity testing of staphylococcus species.
02 October 2020 6,996 7 View
We are working on getting pure desired gene transfected virus. We used YFP fluorescences tags to distinguish the virus with or without gene replaced. Using PCR testing, we always have wild type...
01 August 2020 2,087 1 View
I am interested in understanding more about NGOs that work with refugees. I am brainstorming a project that does not involve IRB approval.
31 July 2020 1,854 5 View
I got an email from someone I do not know saying my name is on her conference presentation. How do I remove it? We have the same first initial and last name (although now she has a different last...
12 January 2020 2,877 4 View
Hi all, I have a rather naive Molecular dynamics question, though functionally useful. I am simulating a rather large structure on a congested HPC, and was wondering if there is an accepted...
30 November 2019 9,877 2 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
02 March 2021 6,270 3 View
I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why
02 March 2021 1,388 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View