I need to do Codon-optimized in the reverse translation part.
In swat, epic, or any other models to calculate inorganic phosphorus cycle, why inorganic phosphorus in active pool can be transfered to stable pool, but inorganic phosphorus in labile pool can...
28 February 2024 1,530 4 View
I am docking a protein structure at different pHs. After obtaining the PDB structure, I protonate the protein at different pHs using pdb2pqr, download the structure, then use Autodock vina to dock...
07 February 2024 6,446 0 View
Hi all, We have a lentiviral vector with a gene of interest that we would like to express. There is no requirement for stable cell line construction, so we will only be transfecting transiently....
29 November 2023 2,802 6 View
I have a spectrum CSV file and I have tried using the spliceCorrection function in R package "prospect" to fix it, but the result is not good. Can anyone please guide me on a method (Software) to...
24 September 2023 1,392 0 View
In most protocols, 50% methanol is used for protein A regeneration, followed by storage, usually in water/buffer/20% ethanol. In my experience, it is critical to apply a 50% methanol wash so the...
29 August 2023 2,511 3 View
Hi all, I am trying to produce an antibody from a plasmid in Freestyle CHOS cells. The plasmid is a HC/furin/p2A/LC vector. The cells were transiently transfected during the exponential phase and...
12 July 2023 2,425 5 View
For a project we needed to use a specific device (Bio-Rad Gene pulser) to electroporate mammalian cells. We set up the instrument with the following settings: Voltage = 1.9kV (we had to use this...
02 July 2023 7,753 3 View
Hello all, I recently started to use peptides for cellular studies. Unfortunately, when I was designing the peptides I did not have a FITC tag to them (the peptide was synthesized by an external...
31 May 2023 1,689 3 View
Hello, We have been trying to insert peptide tags (
07 March 2023 425 6 View
Hello all, I'm using Discovery studio 2019 to dock protein-protein, while "An error was encountered during execution of ViewResults.ds_pl.", then the dock failed. As it works quite well with the...
30 November 2022 829 4 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View
I am optimizing a multi-stage optimization model for disaster response relief delivery. In this regard, I want to know the realistic values of cost parameters related to the operating and setup...
10 June 2024 4,240 3 View
In some practical problems, some equations which are valid under ideal conditions are problematic due to external influences. These equations can lead to large errors in the results. More accurate...
05 June 2024 7,256 3 View
Optimizing Fluid and Electrolyte Management in the Intensive... What are the diagnostic steps and treatment strategies for hyperkalemia in the ICU?
23 May 2024 5,609 1 View
Optimizing Fluid and Electrolyte Management in the Intensive... How does hyponatremia develop in the critical care setting, and what are its clinical manifestations?
23 May 2024 3,532 1 View
Optimizing Fluid and Electrolyte Management in the Intensive... What are some common fluid and electrolyte disturbances encountered in the intensive care unit (ICU)?
23 May 2024 7,791 1 View
Optimizing Fluid and Electrolyte Management in the Intensive... . What are the key principles in the management of hyponatremia, particularly in symptomatic patients?
23 May 2024 2,877 1 View
If I want to put mouse light chain(Sac1 and XbaI) and heavy chain (XhoI and SpeI) in pComb3XSS (phagemid) to form Fab, should I add stop codon at the light chain?
20 May 2024 806 0 View
Unleashing the Potential of C++: Using Optimization Techniqu...
15 May 2024 6,016 5 View
Optimizing Management Strategies for Septic Shock: A Multidi... How does the Surviving Sepsis Campaign guidelines influence the management of septic shock?
12 May 2024 5,779 0 View
