What should I do for my controls not to stain my secondary antibody ( IHC for mice muscle) ?

I am staining mice muscle for macrophages (CD68) and lymphocytes (CD3). The challenge that am facing is that my control is also staining with the secondary antibody even though I did not add the...

15 October 2020 8,139 3 View

Triangulation of data sources in qualitative research: Do you consider it mixed methods research and why, whether its a yes or no?

It is common in qualitative research to interview for example patients and their partners or their health professionals to talk about the same topic and explore the perspectives of all the people...

30 July 2020 538 21 View

How do i determine the sample size for a study looking at the treatment outcomes of mental health patients in a community house ?

How do i determine the sample size for a study looking at the treatment outcomes of mental health patients in a community house that is a step down from inpatient unit. The study will look at...

12 May 2020 7,072 3 View

Who can link me to Cost / Benefit Analysis of Treatment Options for Produced Water of varying quality and quantity?

I work in the oil industry and I wish to focus on reducing reinjection of "free" (non-water right controlled) produced water for beneficial use where economics dictate feasibility. Who can direct...

24 February 2020 3,059 2 View

How is confirmatory factor analysis not just a tautology?

Hello, very new to confirmatory factor analysis (CFA) and structural equation modeling, and I'm trying to understand how to think about confirmatory factor analysis. Suppose I have a construct,...

11 November 2019 3,330 4 View

How to deal with member check conflicts in qualitative research?

Member checking or participant checking is done to validate and improve the credibility of quallitative research results. I am planning to ask my participants to help with that (will ask them to...

27 May 2019 7,799 13 View

Improvised abacus? Are there Latin references to betting at Roman amphitheatres, or modern references to Roman use of abaci? Or was it a gaming board?

11 June 2015 1,010 68 View

Do we have instrument development experts among rehabilitation professionals in Sub-Saharan Africa?

It has been noted that clinicians in Africa are forced to use instrument measures that were developed and validated either for American and European children which are quite limited. It is...

01 January 1970 528 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View